Tag Archives: ucla

Swiss scientists restore voluntary locomotion in paralysed rats.

A study published yesterday in the journal Science, in which a team of scientists led by Professor Gregoire Courtine at the Swiss Federal Institute of Technology used a combination of electrical stimulation, drug treatment and a training regime that encouraged active participation to restore voluntary control of movement in paralysed rats, has received widespread media coverage over the past 24 hours, including reports on the BBC website and ABC news.

If this sounds familiar than it should, as this breakthrough builds on a technique pioneered by Professor V. Reggie Edgerton of UCLA that we reported on last year which enabled a man who had been paralysed in a car accident to stand and take a few steps on a treadmill. Prof. Edgerton wrote an article for Speaking of Research on the importance of animal research to the development of electrostimulation to overcome paralysis.

The key difference between the earlier work and that published by the Swiss team is that whereas in the earlier animal and clinical studies undertaken by Prof. Edgerton there was no conscious control by the rat or human over movement, Prof. Courtine and colleagues devised a training program that allowed the rats to learn to exercise conscious control over the previously paralysed limbs, eventually allowing the rats to run and climb.  In the video below Prof. Courtine discusses the important implications of his team’s work.

There is also an interview available as a podcast without subscription on the Science website in which Prof. Courtine discusses his work in more depth.

The importance of this study should not be underestimated, as it demonstrates that electrostimulation of the lower spinal cord has even greater potential to improve the lives of people with severe spinal cord injuries that was apparent in the earlier studies by Prof. Edgerton and colleagues, studies that were major medical breakthroughs in their own right.

Paul Browne

Best of Friends: University of Texas Professor helps to fund Extremism

Regular readers of this blog will be familiar with the activities of Dr. Steve Best, Professor of Philosophy at the University of Texas at El Paso and long time supporter of animal rights extremism. Indeed, only last month we discussed his support for campaigns of harassment and intimidation against students and scientists, prompted by a recent post on the Southern Poverty Law Center Hatewatch blog which reported on the hate campaign being waged against students by the animal rights extremist Camille Marino.

While Best has been open in his enthusiasm for Marino’s campaigns of harassment and intimidation, and Marino has in turn peppered her “Negotiation is Over (NIO)”website with his videos and essays, he has appeared to limit his involvement to moral support.

Until now…

In a fine report on the online newspaper “Death and Taxes” entitled “Why Is a UT Professor Collecting Donations for an Animal Rights Group that Targets College Professors?” , journalist Carlton Purvis has uncovered evidence that Best’s support for Marino’s campaigns goes well beyond moral support, writing that:

The NIO membership section directs members to a small PayPal button on the right column of the page if they wish to donate. The group also sells annual memberships for $20 and lifetime memberships for $50.  Since that appeal for money, the site has been rapidly pushing out content.”

Why do they need money? Other than website upkeep let us remember that NIO has been offering $100 to anyone who can provide information on biomed undergraduates. See the poster below.

Nonetheless, the article continues:

Click on NIO’s donation button and it takes you to a donation page set up to send money to an account managed by someone using a Road Runner provided email address – the kind that you get for free when you sign up for Internet service.

A quick Google search of the email address reveals the owner of the address, none other than Steven Best, isn’t shy about putting his contact information on everything he touches.”

Oops…providing practical support for a campaign against fellow academics clearly isn’t a good career move for Best, and Marino’s next move proved that they realized this, as Carlton Purvis picks up the story:

Within hours of my email contact with Best on Friday night, the PayPal donation button had been removed from the Negotiation is Over website. Unfortunately, if someone was trying to cover Best’s tracks, they forgot to remove text on the membership page that says, “Please use the Paypal link in the right sidebar of this site or send your enrollment fees through PayPal to sbest1@elp.rr.com.””

DOH!!

The question is now what disciplinary action the University of Texas at El Paso (UTEP) will take against Best for actions, for although Universities are traditionally – and correctly – very keen to protect their staff’s freedom of expression, it is difficult to argue with the view that:

…despite the university’s policy to not get involved with what faculty do on their personal time, it seems like it would be problematic for a university to employ someone who is affiliated with a bounty program that funds harassment targeting university students and faculty.”

We will be watching this developing story with interest, and welcome Carlton Purvis’ tweet that “Rogue animal rights group stops selling memberships after I uncover a #UTEP professor behind the curtain w/this story”.  While we have our doubts about the popularity of NIO memberships, it is always good to see an extremist funding stream closed down.

UTEP President Diana Natalicio will need to think hard about whether her administration can afford to turn a blind eye to behavior directed against other students and staff at other universities that they would never tolerate if it was targeting their own staff and students.

We were also pleased to learn over the weekend that a federal judge has upheld an ordinance that has been critical to UCLA’s efforts to protect its researchers, their families, and their neighbors from harassment by anti–animal research extremists. This ruling makes it clear that there is a difference between legitimate protest and harassment, and shows that society will not stand by and allow citizens to be intimidated and threatened by those who disagree with their work.

All in all a bad week for those who favor harassment and intimidation over dialog and democracy!

Speaking of Research

All in a day’s work: Scientists promote alternatives

Once upon a time, the medication BoTox (made by a company called Allergan) was tested for its potency, on a batch by batch basis, in living animals. This medication, which is really a protein derived from bacteria, has many important therapeutic purposes. For example, it has been shown to be very effective in the treatment of chronic migraine headaches – a condition that can have disabling effects on those who suffer from it. It is used to treat disorders in which people sweat profusely (hyperhidrosis) or have overactive bladders, both of which affect people’s qualities of life by impairing normal social functioning. It has also been used in the treatment of motor disorders like spasticity and dystonia, preventing the irregular and disruptive involuntary movements that are found in these disorders, thereby reducing the physical pain that is so often a consequence of them. Of course, it has also been used for aesthetic reasons, an arguably less compelling medical use.

BoTox is used to treat patients with spastic cerebral palsy, lesseing the pain they suffer as a result of their uncontrolled movements

Because the potency of individual batches of BoTox produced vary, the Food and Drug Administration (FDA) in the United States required Allergan to test each batch on live animals. For each batch, studies were conducted in which the amount of BoTox that was required to produce a specific toxic effect was evaluated in live animals, and the dose was adjusted to ensure that the potency of the drug across batches could be accounted for (roughly, if the batch was half as potent, this can be accounted for by giving twice the dose, ensuring that clinical effects were stable over time). This testing involved a lot of animals, mostly mice.

However, earlier this summer, the FDA changed its mind. It was approached by an organization that had – at considerable expense – developed a test that could determine BoTox potency just as well as the animal tests – but without involving live animals. The test is conducted on cells in a dish.

The organization spent millions of dollars to develop the test and to petition the FDA to consider this replacement for live animal use based upon its empirical results. They were successful.

Who was this organization? Was it the Humane Society of the United States? Perhaps it was People for the Ethical Treatment of Animals, or the Physicians Committee for Responsible Medicine?

It was none of these. Indeed, since none of these organizations spend their operating budgets on the laboratory research that is required to develop alternatives to live animal studies, it couldn’t have been any of them.

So, who accomplished this? It was Allergan itself. Biomedical researchers at the company who used animals in their tests became determined to find a model system that could replace living animals, and they didn’t stop until they found one. They did this though it came at a huge expense to the company. They were committed to producing medicines that people need and to use the fewest animals in the process, and they accomplished that. As the Allergen press release notes, there have been several attempts, using a variety of methods, over the past two decades to develop a replacement for the LD50 test, but until now all these have fallen short.  A report from a 2008 scientific workshop convened by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM)  and the National Toxicology Program Interagency Program for the Evaluation of Alternative Toxicological Methods (NICEATM) provides a good overview of many of the challenges involved in delevoling a replacement for the LD50 test, and the different approaches used to address them.

As always, the alternatives that exist for animal use in biomedical science came from the very scientists who are otherwise roundly criticized by the anti-animal research movement. Maybe the irony is lost on organizations like PCRM, HSUS and PeTA, but not on us. At UCLA, our administration has instituted a funding program that provides seed funding to scientists to promote work on refinement, reduction and replacement. What have the leading anti-research groups done? Nothing, but complain. Perhaps instead of criticizing scientists, these organizations should join with us in attempting to discover alternatives and reduce animal use.

Regards,

David Jentsch

A paralyzed man stands again…thanks to animal research!

Yesterday an article appeared in the New York Times describing how scientists, supported by the National Institutes of Health and the Christopher and Dana Reeve Foundation, have used electrical stimulation of the lower spinal cord to enable a man who had been completely paralyzed below chest level to stand again,  and even to take steps on a treadmill. The good news has since spread around the world, being reported on the BBC, The Times of India, and Canadian TV

While the reports have – perhaps understandably –focused on the fact that this breakthrough has for the first time enabled a man with complete paralysis to stand and take a few steps, the Lancet paper describing this work also reports “improved autonomic function in bladder, sexual and thermoregulatory activity that has been of substantial benefit to the patient”. Such improvements are important as they have a huge impact on the overall wellbeing of a paralysis victim.

In this video Professor V. Reggie Edgerton of UCLA, who lead the team that undertook this study, describes the background to this study, and how discoveries made in both animal and clinical research made it possible.

 

Regular readers of the  Speaking of Research science blog may recognize his name, in October 2009 Prof. Edgerton wrote an article for Speaking of Research  in response to media coverage of a study he and his colleagues had published on the use of implanted electrodes to restore motor function in rats whose spinal cord had been severed, allowing the rats to stand and walk again. This study in rats – which can be read in full in PubMed Central – proved that in the absence of input from the brain due to the spinal cord being severed, electrical stimulation of the caudal segments of the spinal cord could enable the nerve circuits in the lower spine to use input from sensory nerves to control movement, and led directly to the clinical breakthrough reported yesterday.

But as is almost always the case this advance did not come from only one study, many years of basic research in both animals, intitally in cats – where it was first shown that an animal can walk despite the complete transection of the spinal cord -and later in rats, provided the scientific basis for this work, as Prof. Edgerton himself wrote in 2009:

 It has been characterized as a major breakthrough in facilitating the level of recovery of locomotion following a severe spinal cord injury. This in itself implies that these findings were the result of a single experiment with rats. But the reality is that these experiments were based on 100s of other experiments by not only my laboratory, but many other scientists. All of the previous animal experiments relevant to our understanding of the control of movement, involving many different species ranging at least from fish to humans, have contributed to the evolution of the concepts that underlie our most recent publication.”

Only time will tell what this study will mean for the millions paralyzed by spinal injuries – breakthroughs like this are better viewed as the end of the beginning than as the beginning of the end – and much further research will be needed to evaluate and improve this technique before it can be considered for widespread clinical use.

Firstly, the electrode arrray used in this study was relatively basic, but was FDA approved for use in humans and so appropriate for this early clinical study. Prof. Joel Burdick of Caltech, an author on this weeks Lancet study, is working to improve the design of the electrode arrays and the patterns of electrical stimulation applied to the spinal cord. Improvements in the way in which the electrical stimulation is delivered should increase the effectiveness of the technique.

A possible second improvement could be the addition of drugs that activate the locomotor nerve circuts. In the 2009 rat study some animals were treated with agonists for the 5-HT2A and 5-HT1A/7 serotonin receptors – on the basis of earlier research in mice and rats – in addition to receiving electrical stimulation, and it was found that this combination was considerably more effective than electrical stimulation alone. Unfortunately the serotonin agonists used in the 2009 rat study are still experimental and not approved for human use, and so could not be used in the clinical study reported in the Lancet. Hopefully 5-HT2A and 5-HT1A/7 serotonin agonists suitable for use in humans will soon be developed and evaluated in clinical trials, perhaps this weeks result will encourage investment in such drugs.

Many avenues towards repairing spinal cord damage or restoring function are currently being studied, and it is possible that this approach might be superseded in some, or even most, cases by advances in stem cell and regenerative medicine, and of course the various brain machine interfaces that we’ve discussed earlier may prove more appropriate for some conditions and patients.

 For today though, we offer Prof. Edgerton and his team our most heartfelt congratulations on an achievement that gives new hope to thousands.

Let’s also remember that this is but one of many examples of medical progress that animal rights activists would have prevented if they could have.  Fortunately, they did not succeed. It is up to us – medical researchers, health professionals and supporters of science and progress – to make sure it stays that way!

Paul Browne

Waking up the Neighbors: A Neighborhood Response to Animal Rights Extremism

In previous posts, we’ve highlighted revolting new tactics by AR extremists, including the targeting of students and young scientists. Some animal rights extremists envision a future where the nation’s brightest students and talented scientists must live in fear for the safety of themselves and their families.  As for what such war would look like, some of SR’s members have first-hand experience. Now, thanks to some outstanding reporting by Public Television in Southern California (KCET), the public has a chance to see how some scientists who seek to cure disease and end suffering are now the targets arson, assault, vandalism, death threats and stalking.  

The KCET segment exposes the elements of hate and violence in a movement that, paradoxically, believes itself to be based on compassion and kindness.  It makes the main goal of such activism clear: to intimidate, threaten and harass the victim.   As one of the neighbors justifiably asked these activists — “Why don’t you demonstrate at UCLA instead?”   Of course, the answer is obvious; it is easier for these terrorists to threaten families at their homes.   They are not attempting to “educate” anyone about their position.  They are simply trying to force their views on society by violence and threats.

Here’s that report:

Testing the Limits.

Despite their repetitive claims online that their message is welcomed by neighbors, the opposite is actually true. Those who live in proximity of researchers being targeted support their neighbors even though they are, themselves, negatively affected by the focused pickets. This was noted in a report on an animal rights demonstration on a LA activist website which described animal rights extremists being “met with irate neighbors at every visit”.

Recently, near the home of UCLA researcher Edythe London, signs appeared on lawns throughout the neighborhood, with residents trying to give the picketers a strong message. If the petty vandalism and theft of the signs by animal rights protesters is any cue, that message was received.

It isn’t surprising that the animal rights extremists are put out by the clear display of support for scientists by their neighbors, after all, a major objective of “home demonstrations” – aside from harassment and intimidation of targeted individuals and their families mentioned above – is to isolate scientists from their neighbors and turn their neighbors against them. The demonstrations against the UCLA scientists have clearly had the opposite effect, prompting neighbors to rally around the scientists and their families.

SR would like to thank KCET for its balanced look at this issue as the report highlights three important questions that we feel must be answered:

1. How can the topic of animals in research be rationally discussed in the current environment of hate, threats and violence?   How can anyone expect scientists to participate in such discussion if they stand to be targeted at their homes simply for speaking up their minds?

2. How can such a discussion take place when many of those opposed to the research are blind to the countless human and animal lives saved through highly-regulated animal studies?

3. Most importantly, in this toxic environment, how can we ensure continued health advancement when the scientists of tomorrow may become the targets of today?

We believe that the scientific community cannot wait for extremism to end before scientists can start to discuss animal research. We believe that it is no longer acceptable for the scientific community to leave the task of speaking up for science to a handful of brave individuals, we must do more to support and protect those who are targeted by extremists. The answer lies in a community response to extremism that fosters a culture of proactive public education and engagement. Waiting to be targeted before responding is no longer an option, and there are many ways in which students and scientists can discuss the vital role played by animal research in advancing medicine without taking risks, as our friend Scicurious points out in an excellent post on the Experimental Biology 2011 conference:

Many animal researchers are worried about becoming targets for threats and violence, but you don’t necessarily have to stand up and make yourself seen. You can work through your professional societies to talk to people in government. You can write letters to your own government representatives. You can INVITE those representatives into your labs, to see what you do and what it all means. You can go into classrooms and talk about your work, or bring the classrooms to you and show them. You could even write a blog post on the internet. By reposting, retweeting, and passing it on, you can spread the word about funding and the necessity of careful animal research. And if all that still seems too much, you can always start with your family and friends. Tell them about what you do. Many of them may not even know. And tell them what it’s all for, and what we’re going through because of it. Because in this case, when the data speaks in a language only experts can understand, scientists have to stand up and do the talking.”

These are great suggestions, though as the experience of scientists at UCLA shows, in addition to talking to family and friends, talking to your neighbors can yield great results.

Speaking of Research

“Successes of Antivivisection Activists” are delusions, at best

The North American Animal Liberation Press Office drew upon the recent profile of UCLA researchers by the Chronicle of Higher Education in a recent post (warning: extremist website), boasting that information in the article:

validates activists’ tactics and achievements in making the abuse of animals more costly and dangerous for the evil men and women who insist on putting their own careers and greed over the suffering and lives of innocent animals.

What the statement by NAALPO misses, in its simple-minded misstatements of facts regarding research at UCLA, is that biomedical research, including that which involves animal subjects, is going strong on this campus, as it is on others across the world. Certainly, my own research, which in part focuses on the biological mechanisms that mediate addictions, continues unabated. It is humane and responsible, and it progresses. Insinuating that animal rights activists have driven down the number of animals used at UCLA, the NAALPO author indicates that the Chronicle article fails:

…to account for the hundreds of lemur monkeys recently transferred from UCLA to an east coast institution when Lynn Fairbanks gave up her research on them here.

Firstly, lemurs are not monkeys; they are prosimians. Secondly, UCLA never had a colony of lemurs; the animal resource in question is a colony of pedigreed vervet monkeys that now resides at the Wake Forest Primate Research Center. Thirdly, according to Lynn Fairbanks – a member of the Speaking of Research and Pro-test for Science committees,

our partnership with [Wake] has enabled us to expand our research on environmental and genetic origins of psychopathology.

 

Commenting on the move, David Friedman, professor of physiology and associate dean for research at Wake Forest University School of Medicine, who uses behaviorally phenotyped colony animals in his own research on the etiology of alcoholism, told us that:

The vervet research colony was relocated in order to facilitate research teams and institutions working together to concentrate expertise and promote collaboration and to maximize the use of an invaluable animal model for research relevant to a broad range of diseases, including addiction, psychiatric disorders, diabetes, cardiovascular diseases and aging.  Having the colony at Wake Forest facilitates all  these efforts and helps to make sure the resource can be even more widely shared and well utilized than it has been in the past”

Dr. Fairbanks continues making important discoveries by studying these vervet monkeys, discoveries that will contribute to the understanding of the causes of behavioral problems like impulse control disorders, and she remains an important advocate for humane biomedical research on animals.

The three serious failures of fact in this one sentence in the NAALPO statement alone belie the ignorance of the entire posting.

What the NAALPO author also misses is that animal numbers usually decrease when a particular program of research is completed and the resulting discoveries are discussed and translated into clinical research, and ultimately to benefits for human society. They also sometimes decrease when scientists discover new ways to refine their procedures and when technological advances permit reductions in numbers of subjects needed to make discoveries.

The NAALPO author goes on to obsess over the costs of federally-funded research at UCLA and the security measures to protect researchers from violent animal rights extremists. What they seem unable to understand is the cost of not doing research… that a human life – that of your child, your mother or your best friend – has no price tag. These costs are easily justified by the need to ensure that families everywhere are able to live happy and healthy lives.

So, for animal rights extremists in Los Angeles, the delusions of grandeur continue, but the successes are few. Dario Ringach puts it best when he says:

Science is a community effort.  My suspension of animal research does not mean the work has stopped.  It is being performed elsewhere by hundreds of talented colleagues.

Basic discoveries that enable the developments of treatments or cures are emerging at a rate never before seen, and this will continue despite efforts of misguided and hate-mongering animal rights activists wherever scientists pursue open discussion of the goals and nature of biomedical research in which they are engaged.

Regards

David Jentsch

Symposium Explores Animal Rights Tactics, Responses

On Saturday April 24, 2010, the American Physiological Society sponsored a symposium on Trends in Animal Rights Activism and Extremism. This event, attended by about 100 people,  was part of the Experimental Biology 2010 meeting, which was recently held in Anaheim, California. In introducing the symposium, session chair Bill Yates noted the importance of animal welfare, and the obligation of human beings to provide for the well-being, humane care, and judicious use of animals in research. However, some individuals reject the notion that research with animal models plays a critical role in advancing our understanding of biological processes and is essential to the search for cures. Some with this belief use tactics such as violence and intimidation to prevent researchers from conducting studies using animals. The intent of the symposium was to inform researchers about the tactics of animal rights extremists and what researchers and their institutions can do to protect themselves and their work.

Bill Yates opens the discussion

UCLA Senior Campus Counsel Amy Blum opened the symposium by explaining what kinds of protected information may be subject to the federal Freedom of Information Act (FOIA) or state open records laws. Animal rights extremists have used information obtained under FOIA to target investigators for intimidation and harassment. While FOIA is a mandatory disclosure statute, certain kinds of information may be exempted from disclosure, such as privileged communications between attorneys and clients; trade secrets or confidential commercial or financial information; personnel and medical files; or information that might endanger a person’s life or safety. Researchers should exercise care in how documents and communications are written to avoid unnecessary disclosure of personal information or intellectual property. This effort may be “difficult in the short run” but will “make your life easier in the long run,” Blum said.

University of Iowa (UI) Attending Veterinarian and Office of Animal Resources Director Paul Cooper reviewed the 2004 Animal Liberation Front (ALF) break-in during which some 400 rats and mice were removed from the facility. Four individuals were involved in that break-in, and they made a video. It shows them dumping animals into plastic storage bins, destroying laboratory equipment; trashing researchers’ offices, and pouring acid over research records. Cooper noted that the animals in the storage bins were clearly having trouble getting enough air and probably died of suffocation. Based upon the ALF video and images captured by UI security cameras before and after the break-in, it was evident that the intruders included someone who was familiar with the facility. ALF break-ins have been rare occurrences, but Cooper’s message was clear: Every research institution has to take its security seriously because while if an ALF break-in can happen in Iowa City, it can happen anywhere.

David Jentsch discusses events at UCLA with symposium participants

David Jentsch, a UCLA professor of psychology and psychiatry and bio-behavioral sciences, reviewed the history of animal rights extremism at UCLA. From 2001 to 2003, there were annual demonstrations where animal rights demonstrators criticized the university, researchers, and their work. “When they do that and you make no response, you are contributing to the decline in public confidence,” Jentsch noted. Starting around 2003, extremists began sending threatening emails and vandalizing researchers’ homes during late-night visits, which led to a climate of increasing fear. Extremists left a Molotov cocktail on the doorstep of one UCLA researcher—except that they actually left it at the home of the researcher’s elderly neighbor (Fortunately, the device failed to detonate). Another faculty member and his family were subjected to repeated home demonstrations and threats. The university’s only public comment during this period was a statement denouncing terrorism. This was consistent with views widely held across many institutions that they should not respond to accusations against researchers because that would add to the critics’ credibility. It was the university’s pursuit of this strategy of silence in the face of increasingly hostile and violent attacks that ultimately precipitated a crisis: In the fall of 2006, a researcher who was studying how the brain processes visual information announced that he would terminate his research program. He asked in return that animal rights activists leave him and his family alone. He delivered his plea in an email message to the North American Animal Liberation Press Office with the subject line “You win.”

Over the next couple of years, the University’s responses improved, however the activists’ attacks did not abate. In 2007, there was an unsuccessful attempt to firebomb one faculty member’s car, the home of another faculty member was deliberately flooded. In 2008, the door to the same individual’s home was set on fire; a commuter van belonging to the university was burned; and cars were vandalized in the driveway of a post doc’s home and at the home of a researcher’s neighbor. Finally, in early 2009, Jentsch’s car was firebombed in the driveway of his home. This “intensification to a climax of violence” demonstrated to Jentsch that the “strategy that the university was using wasn’t working and wasn’t going to work.” His response was to found Pro-Test for Science, an organization that subsequently staged the first major public demonstration in support of animal research in the United States.

The first Pro-Test for Science Rally was held April 22, 2009. The goal of the rally was to let the public know that “animal research is contributing to basic science understanding of physiology and helping us to solve an array of problems in biomedicine.” Although counter-protesters showed up to take pictures, Jentsch said that not only did this fail to intimidate the participants, it was “fair to say that everyone who came left feeling that there was something they can do” to support research. It should further be noted that since the 2009 Pro-Test rally, there have been no further violent attacks against UCLA researchers.

“Get ahead of the issue,” Jentsch urged. “Don’t wait.” He recommended that every individual scientist get into the habit of engaging the public about science: “Tell them what you do—be your own advocate.”

Hayre fellow Megan Wyeth emphasizes the importance of public outreach

Americans for Medical Progress Hayre Fellow Megan Wyeth spoke about public outreach for the early career scientist. Public outreach can take many forms, she noted, recommending that everyone work within his or her own comfort levels. She urged those who teach to cite the basic animal research that led to the breakthroughs in order to raise their students’ awareness of what animal research has contributed. “Tell people what you do,” Wyeth said. She suggested emphasizing that animal research is necessary for medical progress, that is irreplaceable for the foreseeable future, and that it is a humane and highly regulated activity.  This was a point that was appreciated by many attendees, including session chair Bill Yates who had earlier stressed the importance of developing good relationships with local journalists and conveying this positive message before a crisis occurs.

Alice Ra’anan

Director of Government Relations and Science policy

The American Physiological Society

Bill J. Yates

Chair, Animal Care and Experimentation Committee

The American Physiological Society

Public outreach is an important duty for all involved with medical research, though as Megan said it takes many forms. Allyson Bennett has discussed how scientists can become involved in debates and web-based advocacy , and organize community outreach programs, while Paul Browne has stressed the need for scientists and physicians to explain how animal research has contributed to the latest advances in medicine. There are many ways to improve public understanding of the importance of animal research to medical progress, but they can all be summed up by David Jentsch’s call to “Tell them what you do—be your own advocate”.

Finding animal research in medical news

One of the things that often strikes me when reading about medical advances or clinical trials is how variable the reporting of basic and applied research, including animal research, that underpins the clinical research is.  In some cases it is discussed in some depth, but far too often it is either skimmed over or not mentioned at all.  This is a shame since it makes it more difficult for readers to make the connection between what is happening in the clinic and animal research that may have begun years earlier. A few stories in the news this week illustrate this variability very nicely.

I’ll start with an excellent report by Miriam Falco on CNN entitled “Stem cell treatment goes from lab to operating room” which describes a clinical trial of fetal stem cells in the treatment of Amylotrophic Lateral Sclerosis (Lou Gehrig’s disease), a progressive neurodegenerative disease affecting the motor neurons that leads to severe muscle weakness and eventually death as the muscles that control breathing fail.  As the CNN report points out research on rats was vital to the identification of the correct type of cells for this transplant, and Dr. Eva Feldman demonstrated that injecting fetal stem cells into rats with ALS preserved the large motor neurons and muscle strength.

Lead researcher Dr. Eva Feldman, a neurologist at the University of Michigan, designed the trial just four years ago. After a lot of animal testing, her team determined that using fetal nerve stems rather than human embryonic or adult stem cells (such as bone marrow stem cells) was most effective, she says.

Stem cells have the ability to turn into different cells in the body. However, human embryonic stem cells, which come from 4- or 5-day-old embryos, also been found to sometimes turn into cancer cells. Fetal stem cells, such as those used in this trial, are a few weeks older and have already taken on a specific identity — in this case nerve cells.

Feldman says the fetal stem cells used in this trial did not become any of the unwanted cell types. “That’s very, very important,” she says.

Basic animal research showed the potential of this therapy, but applied research also played an important part in making this clinical trial possible. Through studies on pigs Dr. Nicholas Boulis developed an apparatus that allows the stem cells to be injected at precise locations in the spine, and then practice the technique before attempting to use it on a human patient.

Animal testing also proved very useful when it came to figuring out how to actually inject the stem cells. Emory University’s neurosurgeon Dr. Nicholas Boulis invented the device that holds the needle that injects the stem cells. The goal is to inject the cells without injuring the spine and causing even more paralysis. He practiced on 100 pigs before attempting the procedure on a human.

Our second report is from the LA Times, and in an article entitled “A personal fight against a lethal childhood illness reports on the work being done at the Centre for Duchenne Muscular Dystrophy at UCLA. It’s a nice report which shows how passionate scientists like Stan Nelson and Carrie Miceli are about finding effective treatments and cures for serious diseases.  While the report does refer to  experimental therapies such as exon-skipping and gene therapy it unfortunately does not discuss them or the research that led to their development in any depth.

Exon skipping is a particularly innovative approach to treating some cases of Duchenne Muscular Dystrophy (DMD) where the disease is due to a mutation in the dystrophin  gene that stops translation from messenger RNA prematurely and prevents the production of the protein  dystrophin. In exon-skippping a molecule known as an antisense oligonucleotide or morpholino acts to remove the portion of mRNA that contains the mutation and allows the translational machinery of the cell to read through and produce a working dystrophin protein.  As I discussed in an article last year research in mice and dogs has been crucial to the development and refinement of exon-skipping and early versions of this therapy have already had promising results in clinical trials undertaken at  Great Ormond Street Hospital in London and Royal Victoria Infirmary in Newcastle.  Gene therapy, where the faulty dystrophin gene is replaced by a working version, is also being developed, though it has not yet entered human clinical trials. A recent review (1) available to read for free at PubMed Central discusses the progress that has been made in recent years, the challenges that remain before DMD can be cured, and the vital role played by animal models  in overcoming these challenges. The review also covers stem cell therapy for DMD, another exciting approach to treating the disease that we have discussed previously.

The final news item is a BBC report on a successful clinical trial of stem cells to treat Multiple Sclerosis, this time using stem cells isolated from a patient’s own bone marrow. Multiple Sclerosis (MS) is an autoimmune disorder where the patient’s immune system turns on the myelin sheath that insulates the axons of nerve cells, leading to a range of often serious neurological problems.  At present few effective treatments have been approved for MS, and several are currently being evaluated in clinical trials.  While the improvements seen in the clinical trial were modest they do hold promise for longer and lager trials that are now being planned, and I suspect that as with other therapies the key might be to start treatment early to prevent damage as well as allowing damage to be repaired.

The symptoms of Multiple Sclerosis. Image courtesy of Mikael Häggström

The trial at Frenchay Hospital in Bristol built on years of careful animal research, including research conducted by Professor Neil Scolding who lead this clinical trial.  Interestingly the research, conducted in mice with experimental allergic encephalomyelitis that reproduces many of the features seen in autoimmune diseases that attack the myelin sheath, showed that rather than replacing the damaged cells that produce the myelin sheath or nerve cells the injected stem cells protected the myelin sheath and nerve cells by turning down the pathogenic immune response responsible for damaging the myelin sheath (2,3). This was important since it meant that it was not necessary to inject the stem cells directly into the site of the MS lesion, rather the cells could be as (if not more) effective if injected into the bloodstream so that migrate to tissues such as the lymph nodes where they can interact with cells of the immune system.  This discovery paved the way for the clinical trial reported by the BBC.

There’s a lot of stories in the news that are relevant to animal research, the trouble is that it’s not always easy to see the connection. At Speaking of Research we believe that the onus is on scientists to make sure that when they talk to reporters they give the full picture of what their research involves, and what earlier studies it depended on. Only then can the public really begin to appreciate just how important animal research is to continued medical progress.

Paul Browne

1)      Wang Z. et al. “Gene Therapy in Large Animal Models of Muscular Dystrophy” ILAR J. Volume 50(2), Pages 187-198 (2009). PMCID: PMC2765825

2)      Matysiak M. et al “Stem cells ameliorate EAE via an indoleamine 2,3-dioxygenase (IDO) mechanism” J Neuroimmunol. Volume 193(1-2), Pages 12-23 (2008) DOI:10.1016/j.jneuroim.2007.07.025

3)      Gordon D . et al “Human mesenchymal stem cells abrogate experimental allergic encephalomyelitis after intraperitoneal injection, and with sparse CNS infiltration.” Neurosci Lett. Volume 448(1), Pages 71-73 (2008) DOI:10.1016/j.neulet.2008.10.040